PETRI DISH LAB EXPERIMENT (FIRST LAB)
06SEP11 - 08SEP11
Pictured above is our Petri dish for our sample taken from the bottom of a shoe (Rain Boot). The specimen shown illustrates two separate bacteria colonies, after two days in the incubator (of growth). Below are 3 images taken from the same sample under magnification under a microscope at various levels of magnification. Notice the two different colors of the two separate colonies.
Color: Cream & yellow
Elevation: Raised, Crateriform, flat, convex, hilly
Form: Circular and irregular
Margin: Undulate
Appearance: mostly Opaque and very little translucent
Our objective was to isolate and create a pure culture of a single bacterial colony by using the Streak Plate Method. After we examined and documented what kinds of colonies we had in our Petri dishes, we sterilized an inoculating loop, picked a single colony from our Petri dish using the loop and transferred the colony onto another Petri dish that contained an agar medium. Using the loop we spread the colony onto the agar into four quadrants. We placed the new petri dish into the incubator to be examined next week, and the original Petri dish was placed in the refrigerator.
Conclusion: Our lab experiment demonstrated the growth and quality of the various bacteria found from our sample. It is perhaps surprising to learn how quickly bacteria can grow / and all the places of which it can be found.
Don Caldwell
Melanie Seitz
